goat anti human biotinylated trem2 detection antibody  (R&D Systems)

Journal: bioRxiv

Article Title: SHIP1 regulates TREM2 signalling and macrophage functions in a hiPSC-derived model

doi: 10.1101/2025.07.02.662745

Figure Lengend Snippet: Parent and SHIP1 KO macrophages were activated for 5min with IgG isotype control (IgG ctrl) or the TREM2-activating antibody AF18281 (TREM2 antibody), both at 5 µg/ml. The resulting phosphorylation of downstream signalling molecules (A) SYK, (B) PLCγ2 and (C) Akt was assessed by HTRF. (D) Representative western blot showing expression of TREM2 in wild-type (WT) parent macrophages compared to SHIP1 KO macrophages (KO). (E) Total TREM2 expression, normalised to GAPDH expression, in SHIP1 KO macrophages was quantified by western blot, normalised to expression in parent macrophages. (F) 18h conditioned media was collected from untreated parent and SHIP1 KO macrophages and assessed by ELISA for levels of soluble TREM2. Data are N=4 (A, C, E & F) or N=3 (B), presented as mean ± SEM. Statistical significance of data was assessed by two-way ANOVA with Šidák’s multiple comparisons test (A-C) or unpaired t-test (E & F). *P<0.05, ***P<0.001, ****P<0.0001, ns = not significant.

Article Snippet: Plates were then incubated with 1.5 μg/ml biotinylated TREM2 detection antibody (BAF1828, R&D Systems) for 1h at RT, and then 41.67 ng/ml HRP-conjugated streptavidin (N100, Thermo Fisher) for 1h at RT, washing between these stages.

Techniques: Control, Phospho-proteomics, Western Blot, Expressing, Enzyme-linked Immunosorbent Assay

Journal: Genome medicine

Article Title: TREM2 splice isoforms generate soluble TREM2 species that disrupt long-term potentiation.

doi: 10.1186/s13073-023-01160-z

Figure Lengend Snippet: Fig. 1 TREM2 isoforms expression increases in Alzheimer’s disease. A Expression analysis of TREM2 isoforms (TREM2230, TREM2222, and TREM2219) in different brain regions in control subjects (Control) and patients with late-onset Alzheimer’s disease (AD) using the AMP-AD dataset. B Design of qPCR primers to specifically detect TREM2 isoforms. C qPCR expression analysis of TREM2 isoforms in middle frontal gyrus tissue of control subjects (C) and patients with Alzheimer’s disease (AD). Statistical analysis was performed by the Student t-test for TREM2230 and Mann-Whitney test for TREM2222 and TREM2219. D qPCR expression analysis of TREM2 isoforms in brain cortical tissue of 7-month-old control and 5xFAD mice expressing human TREM2 gene (B6hT2and 5xFADhT2). Animals from both sexes were analyzed together (3 males and 1 female B6hT2; 3 males and 3 females 5xFADhT2). Statistical analysis was performed by the Student t-test. Data are expressed as mean values ± SEM (**P < 0.01; ***P < 0.001)

Article Snippet: Wells were washed 4 times with PBST and incubated with samples diluted in 0.5% BSA in PBST for 2 h at RT, followed by incubation with 0.25 μg/ml of the human TREM2 biotinylated detection antibody (R&D Systems, BAF1828) for 1 h at RT.

Techniques: Expressing, Control, MANN-WHITNEY

Journal: medRxiv

Article Title: MS4A4A modifies the risk of Alzheimer disease by regulating lipid metabolism and immune response in a unique microglia state

doi: 10.1101/2023.02.06.23285545

Figure Lengend Snippet: A. MS4A4A and TREM2 reside on the plasma membrane in microglia. Prior work demonstrates that rs1582763 is associated with elevated CSF sTREM2 and reduced AD risk (termed: protective) and rs6591561 is associated with reduced CSF sTREM2 and increased AD risk (termed: risk) B. Trans-eQTL analyses for rs1582763 and rs6591561 were performed using data derived from tissue samples representing three cortical areas in three independent cohorts (ROSMAP, frontal; Knight ADRC, parietal; Mayo, temporal). C. Trans-eQTL analyses were performed in human microglia clusters from single nuclei RNA-seq. D. Functional genomics were applied to define the impact of rs1582763 and rs6591561 on microglia function using iTF-microglia. E. Summary of major findings.

Article Snippet: For detection, wells were incubated with a Trem2 biotinylated detection antibody (R&D Systems, BAF1729, [0.25μg/ml]) for 1 hour at RT on an orbital shaker.

Techniques: Membrane, Derivative Assay, RNA Sequencing Assay, Functional Assay